Thursday, November 12, 2009

Colgate Soothe N Seal

Male infertility is likely to double, alarm from Milan

For experienced emergency genital diseases of the future: a guide for families of the San Paolo

LONDON-A bomb. For specialists in Milan genital diseases and male sex are an underwater world that threatens to double the incidence of infertility among young people. Boys, abolished the visit of the lever, get the diagnosis of these diseases when it is too late. Result: boom and infertile carriers of sexually transmitted diseases that might infect the partner. The warning is the endocrine-andrologist Mario Mancini, head of the surgery of pediatric hospital Andrology San Paolo in Milan. The expert talks to the data in hand: "From our surveys in high schools in the city shows that a second adolescent does not use a condom. 25% of these came into contact with infections and 12.5% \u200b\u200bturns into "infector". But the situation is no different even in the rest of Italy. "

INFECTIONS AND MORE - There is a universe of andrological diseases that comes out: varicocele and testicular problems, sexually transmitted diseases, tumors. "We could be facing the real emergence of the future. Other than influenza, "warns Mancini. Diseases which, if not diagnosed in time, lead to infertility, "We realized that a young infertile three shows signs of damage from chronic prostate infection. Which makes us think that their infertility was preventable. "

the booklet - The experts of St. Paul are so worried that, in collaboration with the Council of Zone 6 (the area in Milan where the hospital is located), have decided to launch a handbook: "The world under the ' navel-Andrology of the boy, "and distribute it in the study of general practitioners and pediatricians. A dozen pages in which, with simple and direct language, the specialists offer adolescents and their families with information useful to detect possible problems. Objective: To support prevention and early detection. The booklet is particularly aimed at providing adolescents and their families all the information needed to understand se per un particolare problema andrologico c'è bisogno di chiedere l'aiuto del medico di famiglia, del pediatra o dello specialista, oppure se rientra in una situazione di normalità.

Origine:corriere.it
Test Infertilità Maschile

Tuesday, November 10, 2009

What Can I Give 5month Old For Cough

Generation of DNA profiles from fabrics without DNA extraction

Abstract...

DNA profiles can be obtained from fabrics where a person has made direct contact with clothing. A standard approach is to cut out a section of the fabric and then use a commercially available method to extract and isolate the DNA. Alternative methods to isolate DNA include the use of adhesive tape to remove traces of cellular material from the fabric prior to extraction. We report on a process to obtain full DNA profiles using direct amplification from a range of fabrics. The absence of an extraction step both reduces the opportunity for contamination and reduces the loss of DNA during the extraction process, increasing the sensitivity of the process of generating a DNA profile. The process does not require the use of commercially available extraction kits thus reducing the cost of generating a DNA profile from trace amounts of starting material. The results are in part dependent upon the nature of the fabric used to which the DNA has been transferred.

Origine:fsigenetics.com

Megalodon Still Exist D

Casework testing of the multiplex kits AmpF ℓ STR ® SEfiler Plus ™ PCR amplification kit (AB), PowerPlex ® S5 System (Promega) and AmpF ℓ STR ® MiniFiler ™ PCR

Abstract

The STR kits SEfiler Plus™ (D3S1358, FGA, D8S1179, D18S51, D21S11, TH01, VWA, SE33, D2S1338, D16S539, D19S433, and Amelogenin), PowerPlex® S5 System (D18S51, D8S1179, TH01, FGA, Amelogenin) and MiniFiler™ (D13S317, D7S820, Amelogenin, D2S1338, D21S11, D16S539, D18S51, CSF1PO, and FGA) were comparatively tested for their robustness and sensitivity. About fifty stains with highly degraded DNA and little DNA quantity served as examination material (e.g. hair with a telogen root, bones, degraded saliva stains on drinking-vessels and skin cell mixtures).

The PowerPlex® S5 with five German-DNA-database (DAD)-systems and the MiniFiler kit with four topical DAD-systems and further STR markers show reduced amplicon lengths. The SEfiler Plus™ kit represents no MiniSTR multiplex, but contains the nine current DAD-systems and further three systems D2S1338, D16S539, D19S433, which are the potential expansion markers for the German-DNA-database.

We have detected on the basis of our comparative stain investigations, that the SEfiler Plus kit was less sensitive than the PowerPlex® S5 and the MiniFiler kit. The MiniFiler™ and the PowerPlex® S5 kit showed a comparative high sensitivity.

Especially in analysing skin cell mixtures, the MiniFiler kit contained larger differences with regard to the performance of the fluorescent dyes/primer concentration co-ordination than the PowerPlex® S5. The SEfiler Plus kit generated – just as both MiniSTR kits – relative robust typing results, but there appeared an increased sensitivity for “allelic drop-outs” and “imbalances”. Since the SEfiler Plus kit was not planned as MiniSTR concept, “allelic drop-outs” were observed, as expected, more frequent in typing stains with degraded DNA and little DNA quantity, especially in the long PCR products (e.g. D18S51).

Origine: fsigenetics.com

Amature Surgeon Xmas App

Developmental validation of the PowerPlex ® 16 System HS: An improved 16-locus fluorescent STR multiplex

Abstract....

STR multiplexes remain the cornerstone of genotyping forensic samples. The PowerPlex® 16 HS System contains the core CODIS loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA. Additional loci amplified in the multiplex reaction are the sex-determinant locus, amelogenin, and two pentanucleotide STR loci, Penta D and Penta E. The PowerPlex® 16 HS System is an updated version of the PowerPlex 16® System; while the primers and dyes remain unchanged, it introduces an enhanced buffer system that includes hot-start Taq DNA polymerase and ensures robust performance. Due to the modification of the reaction mix, a multi-laboratory developmental validation study was completed to document performance capabilities and limitations for the revised assay. Data within this validation was generated by eight laboratories and served as the basis for the following conclusions: genotyping of single-source samples was consistent across a large range of template DNA concentrations with most laboratories obtaining complete profiles at 62.5pg. Mixture analyses showed that over 90% of minor alleles were detected at 1:9 ratios. Optimum amplification cycle number was ultimately dependent on the sensitivity of the detection instrument and could be adjusted to accommodate a range of DNA template concentrations. Reaction conditions including volume and annealing temperature as well as the concentrations of primers, Taq DNA polymerase, and magnesium were shown to be optimal and able to withstand moderate variations without affecting multiplexed STR amplification. Finally, data from non-probative samples and concordance studies showed consistent results when comparing the PowerPlex® 16 HS System with the PowerPlex® 16 System as well as other commercially available systems.

Origine:fsigenetics.com

Can You Oovoo With Macbook Camera ?

"You can falsify And the alarm for DNA



Think of two brothers and a wealth jealously guarded by her father. One of the children known to be the most reckless, to get their hands on the money, do not hesitate to kill. It attempts to drop the blame on the other. To shed light on the crime, nothing better than to resort to DNA tests. But, surprise, the result just nail the guy angelic and unexpected: the few drops of blood found by police belong to him. The accusation has no more doubts. The jury seems to conduct a conviction announced. Yet, the miscarriage of justice lies in wait.

How come? The genetic evidence is considered the queen, the one that gives an almost infallible certainty involvement in a crime or helps to support the strangeness of the scene of the crime. Since it was introduced (the American FBI began using in 1988, his "invention" dates back four years earlier) has solved many cases and many obscure events of the past thanks to a re-reading techniques are then unavailable. The DNA tests, now famous thanks to TV, film and literature, he also saved the lives of 17 death row inmates in the United States, found innocent after being sentenced through the use of genetic analysis. The improvement of technology today promises greater accuracy and in a short time, perhaps next year, the American police can rely on devices able to read near real-time chromosomal profile of people detained.
The privacy issues are well known. But a recent scientific paper by researchers at a private company and the Israeli police seem to have dealt a severe blow to the ability to maintain the DNA as the sole and indisputable element of incrimination in a criminal trial.

has been shown that it is feasible to produce indistinguishable genetic tests in the laboratory, even without possessing organic material of the person in question: one has access to the "tab" of that individual kept in a database, either legal or medical. And the task is within reach of a biologist with no special skills. "You can easily manipulate the scene of a crime," he told New York Times <+corsivo> <+tondo> Dan Frumkin, lead author of the study, published in <+corsivo> Forensic Science International: Genetics <+tondo>. The purpose of Nucleix, the company he works for, is not entirely disinterested: the bio-tech company in Tel Aviv has made it a point to your test designed to distinguish true DNA samples from those "built."

Back to the two brothers. Just take a cigarette butt, a hair or a glass from which he drank to extract the DNA of the subject and create a sample to be put on a weapon or a ski mask in form of a sketch of a drop of blood or saliva. Do not even need a special skill, many biologists (if dishonest) may perform a similar operation without the need for special equipment. And could split the "work" anonymously between centers dealing with genetics, making orders over the Internet, sending and receiving the samples in the mail.
The key point is that Israeli researchers have sent weapons and other objects "dirty" laboratory used by the U.S. Justice and none of qualified technicians noticed nothing wrong with looking at the track. In one case, a woman's blood have been removed white blood cells (which contain DNA) were then added and it genetic fragments taken from the hair of a man. This finding, which is subjected to one of the most important centers in the United States, was typed without uncertainty as a male profile.

The findings of Frumkin and his colleagues were judged to be 'disturbing' by the American Civil Liberties Union: "The DNA appears to be more easy to spread onto the scene of a crime of the same fingerprints. And we are building a criminal justice system that increasingly relies on this technical evidence. " Some concern was expressed instead
by Peter Lio, a professor of Bioinformatics at the University of Cambridge. "Difficult" age "and" fragment "the DNA in the way usually looks at the crime scene - says -. Of course, today the technology is progressing so fast and allows more sophisticated manipulations. Requiring additional attention and research. For the benefit of DNA evidence, it should be remembered that it is possible to analyze the genetic profile of a person even mixed with 200 tracks of many individuals. "

agree Lucia Bianchi, Lawyer in Florence and an expert in forensic genetics: "In the case of the brothers, the defense would not have sold easily. We must consider the alibi, the way in which the tracks may have been left at a crime scene. And then there is not only the DNA evidence. Consider, for example, the trajectories of the splashes of blood. The gun must be tweaked to perfection. It is not enough sample with which the fake dirt. In short, counterfeiting seem very difficult. "
The fact remains that the DNA test has lost some 'of its aura of infallibility. Although the counter-move is just the authors of that study. Further analysis may find that the genetic sample is artificial (due to the phenomenon of methylation). But no laboratory in the world to run again ...
Amdrea Lavazza
Source: avvenire.it

Saturday, November 7, 2009

Are You Usually Dry Before Period

HEALTH: NANOPARTICLES DAMAGE DNA at a distance,

(AGI) - London, November 6 - Metal Nanoparticles can damage DNA even without entering cells. The discovery, made by a group of researchers at the University 'of Bristol, not only sheds light on the capacity' of interaction of these specific molecular structures within the body but also provides specific guidance on how these molecules can be used to transport all ' inside the human body drugs. The study also raises important issues about the real security for the health of these nanoparticles that are used now a bit 'everywhere, from sunscreens to electronic products. Although, it must be said, the researchers used in their experiments a quantity 'of very high nanoparticles that can hardly' be comparable to that which can 'normally come into contact person using these objects.
article published in the journal Nature Nanotechnology, researchers have exposed some cell cultures in a particular type of nanoparticles made of cobalt chrome. Among the cell culture and nanoparticles have interposed a special protective barrier formed by fibroblasts. At the end you and delle'esperimento 'Potu verify that the DNA of cultured cells was however modified by a cascade of chemical signals induced by fibroblasts, which were actually in direct contact with nanoparticles. "This research - Ashley Blom explained lead author of the research - we understand how they can interact with the body also natural nanoparticles, such as prions that probably use the same mechanisms to interact with DNA.

Source: agi.it

Is Colgate Soothe N Seal Discontinued

HEALTH STUDY: FERTILITY ', DNA TEST REVEALS THE' BIOLOGICAL CLOCK '

HEALTH: FERTILITY ', DNA TEST REVEALS THE' BIOLOGICAL CLOCK '


(ASCA) - Roma, 6 Nov - A genetic test that can read''biological clock''of women and to predict when it will be 'fertile or if and' at risk of premature menopause. The researchers are developing the Center for Human Reproduction in New York, hoping to be able to use within a year. In normal cases a woman has a sharp decrease in the number of oocytes after age 35 until menopause. In 10% of cases, however, 'the decline already occurs' within thirty years. The new examination will 'be able to predict these cases, so as to enable mothers''at risk''to decide whether to anticipate pregnancy or freeze some eggs to increase the possibility 'of future conception.

noe / sam / ss
by asca.it